Transcriptional profiling in burbot (Lota lota) from Lake Mjøsa-A Norwegian Lake contaminated by several organic pollutants
Identifieur interne : 000163 ( Main/Exploration ); précédent : 000162; suivant : 000164Transcriptional profiling in burbot (Lota lota) from Lake Mjøsa-A Norwegian Lake contaminated by several organic pollutants
Auteurs : P L A. Olsvik [Norvège] ; Vidar Berg [Norvège] ; Jan L. Lyche [Norvège]Source :
- Ecotoxicology and environmental safety : (Print) [ 0147-6513 ] ; 2013.
Descripteurs français
- Pascal (Inist)
- Wicri :
English descriptors
- KwdEn :
Abstract
Lake Mjøsa is the largest freshwater repository in Norway, receiving runoff from a wide surrounding region of urban country. As a result of industrial activity, large quantities of persistent organic pollutants (POPs) have been discharged into Lake Mjøsa during the last century. The levels of PCBs, DDTs and PBDEs in burbot from Lake Mjosa (study population) exceed the corresponding levels in burbot from Lake Losna (reference) by a factor of 3, 6 and 113, respectively. We used shotgun and suppression subtraction hybridization (SSH) cDNA libraries followed by 454 FLX sequencing (957 303 reads sequenced in total) and RT-qPCR to study the effects of POPs in burbot from Lake Mjøsa. The gene list of putatively higher or lower expressed genes in liver of burbot from Lake Mjosa compared to liver of burbot from Lake Losna, generated from the SSH cDNA libraries, suggest that mechanisms associated with drug metabolism and oxidative stress are enriched in burbot from Lake Mjosa (Ingenuity Pathway Analysis (IPA) top networks). According to the IPA analyses, the top toxicity list ranking was "LXR/RXR activation", "Negative/Positive acute phase response proteins", "LPS/IL-1-mediated inhibition of RXR function" and "FXR/RXR activation". Functional analyses further identified PPAR, HNF4A, dexamethasone and p-estradiol as potential upstream key regulator factors. Overall, the study shows that SSH cDNA libraries coupled to next-generation sequencing (RNA-Seq) may be a valuable supplement or alternative to microarray technology in toxicogenomic discovery of environmental samples.
Affiliations:
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Lyche</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Department of Production Animal Clinical Science, Norwegian School of Veterinary Science</s1><s2>Oslo</s2><s3>NOR</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Ecotoxicology and environmental safety : (Print)</title><title level="j" type="abbreviated">Ecotoxicol. environ. saf. : (Print)</title><idno type="ISSN">0147-6513</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Ecotoxicology and environmental safety : (Print)</title><title level="j" type="abbreviated">Ecotoxicol. environ. saf. : (Print)</title><idno type="ISSN">0147-6513</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bromine Organic compounds</term><term>Ecotoxicology</term><term>Environment</term><term>Flame retardant</term><term>Freshwater environment</term><term>Lacustrine environment</term><term>Lakes</term><term>Lota lota</term><term>Norway</term><term>Persistent organic pollutant</term><term>Toxicity</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Lac</term><term>Polluant organique persistant</term><term>Retardateur flamme</term><term>Brome Composé organique</term><term>Toxicité</term><term>Ecotoxicologie</term><term>Environnement</term><term>Milieu eau douce</term><term>Milieu lacustre</term><term>Norvège</term><term>Lota lota</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Norvège</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Lac</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lake Mjøsa is the largest freshwater repository in Norway, receiving runoff from a wide surrounding region of urban country. As a result of industrial activity, large quantities of persistent organic pollutants (POPs) have been discharged into Lake Mjøsa during the last century. The levels of PCBs, DDTs and PBDEs in burbot from Lake Mjosa (study population) exceed the corresponding levels in burbot from Lake Losna (reference) by a factor of 3, 6 and 113, respectively. We used shotgun and suppression subtraction hybridization (SSH) cDNA libraries followed by 454 FLX sequencing (957 303 reads sequenced in total) and RT-qPCR to study the effects of POPs in burbot from Lake Mjøsa. The gene list of putatively higher or lower expressed genes in liver of burbot from Lake Mjosa compared to liver of burbot from Lake Losna, generated from the SSH cDNA libraries, suggest that mechanisms associated with drug metabolism and oxidative stress are enriched in burbot from Lake Mjosa (Ingenuity Pathway Analysis (IPA) top networks). According to the IPA analyses, the top toxicity list ranking was "LXR/RXR activation", "Negative/Positive acute phase response proteins", "LPS/IL-1-mediated inhibition of RXR function" and "FXR/RXR activation". Functional analyses further identified PPAR, HNF4A, dexamethasone and p-estradiol as potential upstream key regulator factors. Overall, the study shows that SSH cDNA libraries coupled to next-generation sequencing (RNA-Seq) may be a valuable supplement or alternative to microarray technology in toxicogenomic discovery of environmental samples.</div></front></TEI><affiliations><list><country><li>Norvège</li></country><region><li>Østlandet</li></region><settlement><li>Oslo</li></settlement></list><tree><country name="Norvège"><noRegion><name sortKey="Olsvik, P L A" sort="Olsvik, P L A" uniqKey="Olsvik P" first="P L A." last="Olsvik">P L A. Olsvik</name></noRegion><name sortKey="Berg, Vidar" sort="Berg, Vidar" uniqKey="Berg V" first="Vidar" last="Berg">Vidar Berg</name><name sortKey="Lyche, Jan L" sort="Lyche, Jan L" uniqKey="Lyche J" first="Jan L." last="Lyche">Jan L. Lyche</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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